塔里木油田地区地表温度分布规律的研究

选用塔里木盆地外围 1 5个站 36年地表温度观测资料和油田地区 4个短期观测站短期地表温度观测资料 ,应用气候统计方法 ,将油田地区的短期地表温度资料订正到长年同期 ,并加以分析研究。结果表明 ;塔里木油田地区年平均地表温度由周围向油田地区增加。夏季 ( 7月 )最高 ,冬季 ( 1月 )最低。     

Antisense drug targeting with VEGF mRNA designed by computer aid and inhibitory effects on K562 cell line in vitro

AIM:The effective antisense sequences targeted VEGF mRNA with computer software would be screened and designed, and effect of them on growth K562 cells and protein expression of VEGF were studied with experiments.METHODS:Seven antisense sequences were selected and synthesized, which consisted of 18-20 deoxynucleotide acid and were modified with phosphorothioate, according to principle of low free energy of overall △G₃₇ Overall. Cell growth was assayed by trypan blue dye exclusion assay and level of VEGF protien in the media was determined by ELISA.RESULTS:Six of seven sequences were capable of inhibing growth of K562 cells and downregulating the VEGF protein expression significantly, compared with Scrambed control group. It was found that there was a close correlation between low level of overall △G₃₇ and antisense effectiveness (r=0.887,P<0.01).CONCLUSION:VEGF mRNA antisense oliogdeoxynucleotides, which were designed by computer software of RNAstructure, were able to inhibit growth of K562 and its protein expression. The VEGF mRNA may be new target attached by drugs. At same time, the computer aided design is useful methods to obtain the effective antisense.     

杂草对灭生性除草剂百草枯的抗性问题

本文综述了百草枯抗性杂草的发生、发展情况以及抗性机理方面的报道，并就抗性杂草的治理提出几点建议。     

20%多效唑·甲哌Wong微乳剂防止小麦倒伏和增产机理研究

冬小麦二棱期喷施植物生长调节剂20%多效唑·甲哌鎓微乳剂375 mL/hm2,可以显著抑制茎秆基部节间伸长,增加各节间充实度,其中赤霉素(Gas)和生长素(IAA)降低,可显著增强小麦抗倒伏能力和降低田间倒伏率.处理还协调了穗数、穗粒数和粒重的关系,增产幅度6.2%～28.6%.增产原因可能在于促进籽粒灌浆强,增加籽粒中内源Gas、IAA、细胞分裂素(CTKs)的水平,增强了籽粒库活性,同时促进茎叶中干物质向籽粒运转.     

双孢蘑菇性亲和性相关分子标记的初步筛选

以传统的形态，生理生化分析和最新的DCS－PDMA性亲和性测定方法为基础， 结合群体分离分析和RAPD技术来源于同一双孢蘑菇异核体菌株的12个不育同核原生质体个体进行分析，筛选与性亲和性相关的分子标记。研究结果表明，供试的12个不育同核原生质体个体被分成两大类性亲和性类型，其中一类（A^ ）包括不育同核原生质体个体B、C、D、E、F、G、H、I、J、L，另一类（A^-）则仅仅包括不育同核原生质体个体K和M，同时筛选到一个与性亲和性相关的分子标记OPA16 1500。从而为间地利用双孢蘑菇本身特有的交配型作标记来指导杂交育种工作和进一步将性亲和性基因定位分离克隆奠定了坚实的基础。     

苹果柱型基因Co的一个AFLP 标记的SCAR转换

 将苹果柱型基因的一个AFLP 标记成功地转换成了简单实用的SCAR 标记。首先对AFLP 标记片段进行序列测定, 然后根据序列特点设计了两对特异引物CoA1/ CoA2 和CoA1/ CoA3 , 每条引物长20 bp。PCR 结果表明CoA1/ CoA2 可以扩增出216 bp 和148 bp 两条带, 其中216 bp 的为柱型性状的特征带; CoA1/CoA3 可以扩增出273 bp 和205 bp 的两条带, 其中273 bp 的为柱型性状的特征带。两对引物在杂交后代中扩增出的特征带与柱型性状的分离重组率都很低(CoA1/ CoA2 为6. 3 % ±2. 5 %; CoA1/ CoA3 为7. 3 % ±2. 6 %) , 所以它们都可以作为该SCAR 标记的特异引物所用。     

典型光活化毒素α-三噻吩对棉铃虫和亚洲玉米螟Na~+-K~+-ATPase的抑制作用

以棉铃虫 H elicoverpa armigera和亚洲玉米螟 Ostrinia furnacalis为试虫 ,建立 Na+- K+-ATPase最佳反应系统 ,研究典型光活化毒素 α-三噻吩 (简称 α- T)对 Na+- K+- ATPase的影响。棉铃虫 Na+- K+- ATPase活力最佳测试条件为酶源蛋白浓度 6μg/ m L ,反应温度 35～ 4 0℃ ,反应时间6min;亚洲玉米螟则为酶源蛋白浓度 8μg/ m L,反应温度 35℃ ,反应时间 6min。近紫外光照 (30 0～4 0 0 nm )对棉铃虫和亚洲玉米螟离体 Na+- K+- ATPase活力基本没有影响 ,但对活体活力有很强的抑制作用。光照和无光照条件下 ,α- T对两种昆虫离体和活体 Na+- K+- ATPase活力均有不同程度的抑制作用。光照组 α- T对亚洲玉米螟 Na+- K+- ATPase的抑制率高于无光照组 ,且处理浓度或剂量越高 ,其抑制率越大 ;对棉铃虫 Na+- K+- ATPase抑制作用不显著     

Inhibition of K562 cell growth by antisense drug targeting with VEGF mRNA in vitro

AIM: To investigate inhibition of K562 cell growth by antisense drug targeted VEGF mRNA. METHODS: X7, 20-mer antisense sequences were selected, synthesized and modified with phosphorothioate. The drug was transfected into K562 cells in the present of lipofection. Cell growth was assayed by trypan blue dye exclusion assay and MTT. The level of VEGF protein in the media was determined by ELISA. The morphology of apoptotic cells were observed by Giemsa staining, and the propotion of apoptotic cells was detected by flow cytometry. RESULTS: The antisense drug inhibited growth of K562 and downregulated expression of VEGF protein significantly, compared with Scrambed control group and showed dose-dependent relation. Signs of apoptosis of K562 cells were not observed. CONCLUSION: Inhibition of K562 cell proliferation, but not cells apoptosis induction is the mechanism of inhibing growth of K562 cells by antisense drug targeted VEGF mRNA. At same time, VEGF has function of promoting K562 cell proliferation, and VEGF mRNA may be a new target attached by drugs.     

维生素E对绵羊鲜精及冻精精液品质的影响

日粮中添加维生素E可以提高绵羊鲜精的活率 ,对照组和试验组的活率分别为 0 72± 0 0 7和 0 78± 0 0 6(P <0 0 5 ) ,显著改善鲜精精液品质。采用两步稀释法在绵羊冷冻精液稀释液中添加维生素E ,可以极显著降低冷冻对精子顶体的冷刺激损害程度 ,试验组的活率 ( 0 43 5± 0 0 2 0 )极显著高于对照组 ( 0 3 65± 0 0 2 6) (P <0 0 1) ,精子顶体的总异常率试验组 ( 3 3 72 % )极显著低于对照组 ( 4 4 3 5 % ) (P <0 0 1) ,其中试验组顶体膨胀率和脱落率与对照组分别为 1 2 8%±0 5 8%、2 3 0 8%± 1 45 %与 2 68%± 0 5 9%、2 8 96%± 3 14 %。冷冻精液品质显著改善。     

瘦肉型猪(PIC344)精液碱性磷酸酶的分离纯化及部分性质研究

用正丁醇抽提、硫酸铵分级沉淀、DEAE SepharoseFF和SephacrylS 200柱层系,从瘦肉型猪(PIC344)精液中提取出碱性磷酸酶。纯化倍数11 46,比活为81 23U/mg蛋白。提取液经PAGE和SDS PAGE检测,呈现一条带。该酶相对分子质量为90 12KD,亚基分子质量为48 41KD,该酶为两个相同亚基组成。等电点为8 19,最适pH值9 5,最适温度为40℃,以磷酸苯二钠为底物测得Km值为3 98×10-4mol/L。经分析,Cu2 、Cd2 为酶的抑制剂,Ni2 、Ca2 、Mg2 为该酶的激活剂。选用CuSO4、Cd(NO3)2作酶的抑制类型判断,结果表明,CuSO4为非竞争性抑制,抑制常数为1 32×10-3mol/L,Cd(NO3)2为竞争性抑制,抑制常数为3 33×10-4mol/L。